Link to plaintext file of prion amino acid sequences
Link to database-by-domain of gapped and aligned of amino acid sequences

Link to plaintext file of prion DNA open reading frame sequences
Link to database-by-domain of gapped and aligned of DNA sequences

Sequenced Species and Prion Substitutions

Here are the species whose prion sequence is known:

gallus gallus, trichosurus vulpecula, mustela putorius, mustela vison, bos taurus, capra hirtus, ovis aries, bos taurus, odocoileus hemionus, cervus elaphus, tragelaphus strepsiceros, tragelaphus strepsiceros, sus scrofa, camelus dromedarius, cricetulus griseus, mesocricetus auratus, mus musculus, rattus rattus, cricetulus migratorius, mus musculus, rattus norvegicus, oryctolagus cuniculus, saimiri sciureus, ateles paniscus, ateles geoffroyi, callithrix jacchus, cebus apella, aotus trivirgatus, callicebus moloch, cercocebus torquatus atys, cercocebus aterrimus, cercopithecus dianae, cercopithecus neglectus, theropithecus gelada, cercopithecus aethiops, cercopithecus mona, cercopithecus patas, mandrillus sphinx, presbytis francoisi, macaca sylvanus, macaca arctoides, macaca fuscata, macaca fascicularis, macaca mulatta, macaca nemestrina, papio hamadryas, colobus guereza, homo sapiens, hylobates syndactylus, gorilla gorilla, hyalobates lar, pan troglodytes, pongo pygmaeus, symphalangus syndactylus.

These are respectively:

chicken, brush-tailed possum, european polecat, american mink, bovine, long, goat, sheep, bovine, short, mule deer , red deer , greater kudu, greater kudu, pig, arabian camel, chinese hamster, syrian golden hamster, mouse long inc, zitter rat, armenian hamster, mouse short inc, rat, rabbit NZ, squirrel monkey, black spider monkey, bhspider monkey, common marmoset, brown-capped capuchin, night monkey, dusky titi, sooty mangabey, mangabey, dianae monkey , baboon, gelada baboon, green monkey/grivet, baboon, baboon, mandrill, langur, barbary macaque, stump-tailed macaque, japanese macaque, crab-eating macaque, rhesus macaque, pig-tailed macaque, hamadryas baboon, colubus, human, siamang, lowland gorilla, malayan gibbon, chimpanzee, orangutan, gibbon

Cat and oryx were further claimed by W. Goldmann in the 4 July 96 Nature but are nowhere available.

The table below gives summary statistics. One sees that alanine occurs 10 times in the consensus sequence and was substituted a total of 13 times in the 52 species for an average rate of 1.3 times per occurrence or 1.3/52 = 0.025 per species.

total subsresidueoccurenceave subs
13ala101.3
7cys41.8
8asp61.3
9glu81.1
12phe71.7
66gly471.4
9his91.0
15ile81.9
13lys111.2
23leu131.8
21met111.9
27asn141.9
18phe161.1
22gln161.4
12arg101.2
21ser131.6
19thr131.5
22val151.5
12trp91.3
21tyr141.5
16-72.3
386totals2611.5

This table gives, by consensus numbered position, the ancestral amino acid, the number of substitutions,and the amino acid variations found. For example, the third line states that the third amino acid is asparagine, with the variants lysine and histidine also found.

pos	#subs	aa	variations
1	1	m			
2	2	a	v		
3	3	n	l	h	
4	3	l	s		
5	2	-	h		
6	3	-	i	m	
7	2	g	s		
8	4	c	s	y	g
9	1	w			
10	3	m	i	l	
11	1	l			
12	4	v	a	f	l
13	2	l	v		
14	1	f			
15	1	v			
16	3	a	t	v	
17	3	t	m	a	
18	2	w	c		
19	2	s	t		
20	2	d	n		
21	3	l	v	i	
22	2	g	a		
23	2	l	f		
24	1	c			
25	1	k			
26	1	k			
27	1	r			
28	1	p			
29	1	k			
30	1	g			
31	1	g			
32	1	g	-		
33	2	w			
34	1	n			
35	1	t			
36	1	g			
37	1	g			
38	1	s			
39	1	r			
40	1	y			
41	1	p			
42	1	g			
43	1	q			
44	2	g	s		
45	1	s			
46	1	p			
47	1	g			
48	1	g			
49	1	n			
50	2	r	l		
51	1	y			
52	1	p			
53	2	p	s		
54	1	q			
55	3	g	s	e	
56	1	g			
57	2	g	-		
58	5	g	-	t	d
59	1	w			
60	1	g			
61	1	q			
62	1	p			
63	1	h			
64	2	g	v		
65	1	g			
66	2	g	s		
67	1	w			
68	1	g			
69	1	q			
70	1	p			
71	1	h			
72	1	g			
73	1	g			
74	2	g	s		
75	2	w	-		
76	1	g			
77	1	q			
78	1	p			
79	1	h			
80	1	g			
81	1	g			
82	1	g			
83	2	-	g		
84	1	w			
85	2	g	s		
86	1	q			
87	1	p			
88	1	h			
89	1	g			
90	1	g			
91	1	g			
92	2	g	-		
93	1	w			
94	1	g			
95	1	q			
96	1	g	a		
97	1	g			
98	2	g	-		
99	3	t	s	a	
100	1	h			
101	3	n	s	g	
102	1	q			
103	1	w			
104	3	n	h	g	
105	1	k			
106	2	p	s		
107	2	s	n		
108	1	k			
109	1	p			
110	1	k			
111	2	t	s		
112	2	n	s		
113	3	m	l	f	
114	1	k			
115	1	h			
116	2	m	v		
117	1	a			
118	1	g			
119	1	a			
120	1	a			
121	1	a			
122	1	a			
123	1	g			
124	1	a			
125	1	v			
126	1	v			
127	1	g			
128	1	g			
129	1	l			
130	1	g			
131	1	g			
132	1	y			
133	1	m			
134	1	l			
135	1	g			
136	1	s			
137	1	a			
138	1	m			
139	2	s	n		
140	1	r			
141	1	p			
142	3	l	i	m	
143	3	i	l	l	
144	1	h			
145	2	f	l		
146	1	g			
147	3	n	s	p	
148	2	d	e		
149	2	y	w		
150	1	e			
151	1	d			
152	1	r			
153	1	y			
154	1	y			
155	1	r			
156	1	e			
157	1	n			
158	1	m			
159	3	y	h	n	
160	1	r			
161	1	y			
162	1	p			
163	2	n	s		
164	1	q			
165	1	v			
166	1	y			
167	1	y			
168	2	r	k		
169	1	p			
170	2	v	m		
171	1	d			
172	3	q	e	r	
173	1	y			
174	2	s	n		
175	2	n	s		
176	1	q			
177	1	n			
178	3	n	s	t	
179	2	f	l		
180	1	v			
181	1	h			
182	1	d			
183	1	c			
184	1	v			
185	1	n			
186	2	i	v		
187	2	t			
188	2	i	v		
189	1	k			
190	2	q	e		
191	1	h			
192	1	t			
193	1	v			
194	1	t			
195	1	t			
196	1	t			
197	1	t			
198	1	k			
199	1	g			
200	1	e			
201	1	n			
202	2	f	l		
203	1	t			
204	1	e			
205	1	t			
206	1	d			
207	3	v	i	m	
208	1	k			
209	2	m	i		
210	2	m	i		
211	1	e			
212	1	r			
213	1	v			
214	1	v			
215	1	e			
216	1	q			
217	1	m			
218	1	c			
219	3	i	v	t	
220	1	t			
221	1	q			
222	2	y	t		
223	2	e	q		
224	3	k	r	q	
225	1	e			
226	2	s	y		
227	2	q	e		
228	1	a			
229	3	y	a	s	
230	2	y	a		
231	3	q	g	d	
232	2	-	g		
233	1	r			
234	4	g	r	a	-
235	2	s	a		
236	2	s	g		
237	2	-	s		
238	4	m	-	a	s
239	2	-	t		
240	2	v	-		
241	3	-	i	l	
242	3	l	f	-	
243	1	f			
244	1	s			
245	2	s	p		
246	1	p			
247	1	p			
248	1	v			
249	1	i			
250	1	l			
251	1	l			
252	1	i			
253	1	s			
254	2	f	l		
255	1	l			
256	1	i			
257	2	f	l		
258	1	l			
259	2	i	m		
260	1	v			
261	1	g